The next generation 800™ TS Absorbance Reader was recently
introduced in a previous blog posting. The many enhancements to both hardware
and software were designed to simplify workflows for many of the most commonly performed assays. Assays such as ELISA, UV-absorption and colorimetric
absorbance are performed in a variety of laboratory settings ranging from basic
research in academic labs to clinical laboratory testing.
A long studied enzyme family found in a diverse range of organisms includes a group of alcohol dehydrogenase enzymes (ADH) which are responsible for interconversion between alcohols and aldehydes or ketones. The enzymatic catalysis of alcohol requires the reduction of the cofactor nicotinamide adenine dinucleotide (NAD+) to the reduced form NADH or the reverse chemical process for the formation of alcohol.
The spectral properties of the reduced form NADH, show an
absorption peak at 340 nm providing a simple method to monitor progression of a
reaction. The enzymatic activity of ADH
from S. cerevisiae was determined for
the two alcohols, ethanol and 2-propanol, by obtaining kinetic absorbance
measurements at 340 nm. Reaction velocities were calculated using Gen5™ Data
Analysis software; Michaelis-Menten and Lineweaver Burk plots were generated to
determine Km and Vmax for each reaction.
These and similar experiments are made simple by the capabilities of the new, easy-to-use 800 TS. The 800 TS brings excellent performance, high quality and value to many laboratories. It's no wonder that BioTek continues to be the most trusted and well-known name in microplate readers and other life science instrumentation.
By: BioTek Instruments, Peter J. Brescia Jr., MSc, MBA
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