Thursday, June 3, 2010

Take3 Plate goes fluorescent!

Over the course of five application notes, we have demonstrated the utility of the Take3 plate for micro-volume analysis of biomolecules using native absorbance characteristics (A260: nucleic acids; A280: proteins). To achieve greater sensitivity and accurately quantify these biomolecules at lower concentrations, fluorescence labeling and detection is usually mandated.

Using DNA samples and Life Technologies Quant-iT PicoGreen reagent, the Take3 plate proved useful for performing fluorescent micro-volume assays at low ng/mL concentrations. This effectively extended the dynamic range for DNA quantification by approximately three orders of magnitude relative to measurements involving native absorbance at A260.