Thursday, March 1, 2018

Multi-Detection, Imaging and HTS Live Cell Applications Workshop

Recently, I had the privilege of leading a multi-day microplate reading and cellular imaging workshop in Santiago, Chile at the Pontificia Universidad Católica de Chile. My friend and colleague, Dr. Carlos F. Lagos, Assistant Professor and one of the leaders at the Center for Translational Endocrinology (CETREN) in the School of Medicine’s Department of Endocrinology, sponsored the event. The purpose of the workshop was to introduce attendees to kinetic and endpoint methods for monitoring multiple phenotypic cellular responses using microscopy and PMT-based detection.

Over the three day workshop, the group used BioTek’s Cytation 5 Cell Imaging Multi-Mode Reader, BioSpa Automated Incubator and MultiFlo FX Multi-Mode Dispenser to perform several assay procedures, including: Cell proliferation, apoptosis, and necrosis using a 2D cell model; Cytotoxicity and cell viability using 2D and 3D cell models; and 2D scratch wound healing assays.

To learn about other applications enabled with Cytation 5, visit BioTek’s Imaging & Microscopy Applications page.

The group is busy with hands-on assay preparation.
Left: Discussing image capture and analysis.
Right: Workshop attendees and representatives from GrupoBios and Pontificia Universidad Catolica de Chile.

By: BioTek Instruments, Brad Larson, Principal Scientist

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