Friday, January 16, 2015

Flat Stanley…a Model for Cell Culture?


Flat Stanley


I’m sure most, if not all of you are familiar with Flat Stanley. Originally a series of books, he’s now more famous for the projects that are performed in elementary school with him. Students color in a picture, cut it out, and then take him on trips, or mail him to friends or family across the country and the world. The idea is to help kids learn about other people, places, and cultures that they might otherwise not even know existed. A great way to get children to learn in a fun, unique way; wouldn’t you agree?

But would you also agree that Flat Stanley makes a great model of the tissues and tumors that exist within the human body? Preposterous you would most likely say. Yet upon closer examination, drug discovery has been using the “Flat Stanley” method of tissue culture for decades. Cells are added to wells of a slide or microtiter plate, spread out, and attach to the bottom in a flat, two-dimensional manner. Unfortunately this cell culture model has led to numerous clinical failures of lead molecules, as in vitro results do not correlate to what is actually seen in vivo with animal or human subjects. Therefore a new cell culture model is needed. Three dimensional (3D) cell culture is poised to meet this demand. The 3D cell culture methods which now exist, including scaffold-, hanging drop-, or ULA plate-based models, provide a means in which cells aggregate together into structures that have length, width, and depth to them, as they would in the body.

The following links provide information regarding the projects that BioTek has performed, as well as the liquid handling, reading, and imaging instrumentation used to further enhance the results generated using 3D cultured cells.

Application Notes: http://www.biotek.com/resources/app_notes.html
Hot Applications: http://www.biotek.com/resources/hot_applications.html
www.cellimager.com: http://www.cellimager.com/#!applications/cjg9
Presentation: http://www.biotek.com/resources/articles/enabling-3d-cell-culture-methods-and-applications.html

As you will see, BioTek can help you out whether you are new to 3D cell culture, or would like to see what new opportunities exist using cells cultured in this manner.

Move over Flat Stanley…there’s a new cell culture model in town!


By: BioTek Instruments, Brad Larson, Principal Scientist

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