Figure 1. Energy Level Diagram of Raman and Fluorescence Signals.
Figure 2. Comparison of Raman and Absorbance signal IL-6 ELISA Dose Response Curves.
Raman and resonance Raman scattering measurements are usually performed using an expensive dedicated tunable laser based instrument with detection primarily taking place in the IR portion of the spectrum. Sword peroxidase chemistry employs reagents that have emissions in the near IR spectrum that an appropriately configured Synergy H4 Hybrid Multi-Mode Microplate Reader from BioTek Instruments is capable of detecting with high sensitivity. This combination of chemistry and hardware provides an increase in ELISA sensitivity without the additional costs of a dedicated instrument.
By: BioTek Instruments, Paul Held, PhD., Laboratory Manager