The second day of our roadshow brought new opportunities to show Cytation™ 3's versatility and adaptability. Again, we invited participants to test their specific samples on Cytation3. Among the many samples we read and imaged, here’s one that stood out during Day 2:
Sub-population Analysis: Within a mixed cell population, researchers needed to identify those cells that differentiated into cardiomyocytes after exposure to different compounds. We used filters to image and count the cardiomyocytes (red) against the entire cell population (blue) in a dose-response study, even at the lower compound concentrations. Manual counting would have taken almost an hour, but Cytation3 zipped through the cell counts in just a few moments.
While the comparison to manual counting was apparent (and impressive!), many participants wanted to know: How does this compare to confocal and high-end microscopes? Good question! Cytation3 was designed to cover many cell-based applications including those common to fluorescence microscopy and PMT-based assays, all at an affordable price. We chose to provide digital wide-field fluorescence microscopy instead of confocal fluorescence microscopy as a balance between applications coverage and cost. The cardiomyocyte application described above is a core capability provided by Cytation3 in that phenotype changes can be quantified relative to total cells viewed using the sub-population analysis capability of Gen5.
But the Cytation3 is much more than a wide-field fluorescence microscope: it is best described as a combination of a digital wide-field fluorescence and bright-field microscope and multi-mode PMT-based whole well microplate reader.Cytation3 can alleviate bottlenecks in core facilities; allowing for benchtop imaging and immediate results, and freeing up the high end (and much more expensive) microscopes for advanced studies. Additionally, by combining whole well reading and imaging in one unit, users can hit pick assays, where the plate is read, and only the wells of interest are imaged – increasing throughput, saving time and significantly decreasing the data storage space required.
While we can’t share the cardiomyocyte data here, you can check out Cytation3's cell counting abilities in our application titled, Analysis of Nuclear Stained Cells - Using the Cytation™3 Cell Imaging Multi-Mode Microplate Reader with DAPI-Stained Cells.
By: BioTek Instruments