Here we demonstrate the ability to perform automated compound profiling applications for two separate drug target families, known for selectivity issues.
Kinases are one of the most diverse and highly studied gene families. It is becoming increasingly important for compounds to be profiled against other kinases due to the non-specificity of ATP competitive inhibitors. In this application, we used a luminescent assay chemistry which is able to detect small changes in ATP consumption from kinases having large ranges of ATPKm app values. A single set of reagents is used for all kinase/substrate combinations.
By, BioTek Instruments, Brad Larson, Principal Scientist