Thursday, September 24, 2009

Cell Dispensing in 1536-well Microplates

Miniaturization of assay volumes is important to reduce amounts of reagents regents used per assay. This is especially true of cell-based assays, where screening campaigns can demand up to 10exp10 cells. 1536-well microplates can reduce the amount of cells required for screening campaigns, sometimes by as much as an order of magnitude. EL406 is a Combination Washer Dispenser that can be used to dispense cells rapidly and with good precision into these high density plates.

Below is an example of the reproducibility of cell dispensing in the preparation of a label-free, cell-based assay:

an example of the reproducibility of cell dispensing in the preparation of a label-free, cell-based assay

As can be seen from the graph, using an extracellular matrix (ECM) of fibrinogen, CHO cells could be dispensed across a 1536-well microplate with a precision of 6 %CV, as measured by label-free detection. An application note is available depicting the complete work.

 
What density microplates do you use for your cell-based assays? If you are not currently using 1536-well densities, will you in the future?


By: BioTek Instruments

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